WebB, human monocyte-derived macrophages were transfected with control or Akt3 siRNA and then incubated with 0.1 mg/ml LDL for 48 h in the presence or absence of the indicated inhibitors as in A. Lipids were extracted, and total cellular cholesterol was quantified using a Biovision Cholesterol/CE Quantitation Kit II (n = 6). WebNov 27, 2024 · cDNA was synthesized using RT-&GO™ Mastermix (MP Biomedicals, Santa Ana, CA) according to the manufacturer’s protocol. Quantitative real-time PCR was performed using each cDNA with the Light-Cycler 480 SYBR Green I Master (Roche, Mannheim, Germany). ... (BioVision, Mountain View, CA, USA) according to the …
A simple technique for quantifying apoptosis in 96-well plates
WebAug 8, 2012 · Plasma and urinary Ca 2+ quantification. Plasma and urinary Ca 2+ were measured with a colorimetric (Biovision, Mountain View, CA) assay kit according to the manufacturer's instructions using a colorimetric plate reader (Titertek, Huntsville, AL). Absorbance was measured at 570 nm, and values were analyzed with Multiskan Ascent. WebAug 8, 2014 · Glucose and lactate levels in zebrafish brain were assessed using the glucose and lactate assay kit (BioVision, Mountain View, CA, USA) following the manufacturer's protocols. Both, glucose and lactate contents were measured at 570 nm with a Synergy HT spectrophotometer (BIO-TEK) for colorimetric assay. Each sample was assayed in … hill badges
Anoikis Assay kit (ab284938) Abcam
WebView company leaders and background information for Biovision, Inc. Search our database of over 100 million company and executive profiles. ... 980 Linda Vista Ave Mountain … WebArticle Snippet: The activities of caspase-8, -9, and -3 were determined by flow cytometry using the CaspGLOW TM Fluorescein Active Caspase Staining Kit (BioVision, Mountain View, CA), according to the specifications of the manufacturer. Techniques: Concentration Assay, Derivative Assay, Incubation, Staining, Flow Cytometry, Activity Assay WebBioVision rev. 05/11 BioVision Research Products Tel: 650-428-0236 ••• Fax: 650-428-0336 980 Linda Vista Avenue, Mountain View, CA 94043 USA www.biovision.com •••• [email protected] Page 1 For research use only D-Lactate Colorimetric Assay … smart and final 95124