Hoechst excitation

Author: vlzq

August undefined, 2024

WebHoechst 33342 is a fluorescent compound with an excitation peak at 352 nm and an emission peak at 454 nm, giving it a fairly large Stokes' Shift of 102nm. It can be excited … WebSep 9, 2024 · Introduction The World Health Organization has proposed a 25% reduction in premature cardiovascular disease mortality by 2025 (Roth et al., 2015; Joseph et al., 2024). Myocardial ischemia (MI) is a pathological state that prevents normal cardiac function, and is the most common cause of cardiovascular disease.

Triple Band Excitation: DAPI-FITC-TRITC Nikon’s MicroscopyU

WebApr 18, 2024 · Hoechst is widely used in co-staining applications that simultaneously visualize DNA and other cellular structures or specific proteins. Its excitation/emission spectra do not overlap with other commonly used small-molecule fluorophores and fluorescent proteins that emit in the green-red range. WebSep 12, 2016 · R -Phycoerythrin ( R -PE), one of the chemical constituents of red algae, could produce singlet oxygen upon excitation with the appropriate radiation and possibly be used in photodynamic therapy (PDT) for cancer. Documents reported that R -PE could inhibit cell proliferation in HepG2 and A549 cells, which was significative for cancer therapy. country care online hire

Hoechst or DAPI for nuclear staining? - Biology Stack Exchange

WebEpi Red LED Excitation . Turns “ON” or “OFF” the 640 nm overhead Red lighting (for excitation of Cy5, Cy5.5 and Alexa 647) 8 . Epi UV Excitation . Turns “ON” or “OFF” the 365 nm overhead UV lighting (for excitation of QDot, wtGFP, Hoechst Blue and … WebFor imaging Hoechst 33342 staining, a 405 nm laser was used for excitation (solid-state laser rated power = 100 mW) together with a blue 430–470 nm emission filter. Live/dead cell analysis was performed using IN Carta analysis software128 (GE Healthcare). WebHoechst 33258 is slightly more water soluble than Hoechst 33342, but both have been used extensively to stain live cells. The products may be used in fluorescence microscopy, … country care nursing home

Hoechst 33342 - an overview ScienceDirect Topics

Fluorescence - Flow Cytometry Guide Bio-Rad

Web(excitation: 340 ± 6 nm, emission: 470 ± 20 nm) was used for excitation and registration of Hoechst fluo-rescence; for PI, a set of Texas Red light filters (exci-tation: 575 ± 10 nm, emission: 624 ± 10 nm). Cells were counted in two channels in the ImageJ using the Cell Counter plugin. The Hoechst dye enters cells and WebFor Hoechst 33342-DNA the mode of excitation was a mixture of two- and three-photon excitation to the longest excitation wavelength of 910 nm, and we were unable to observe … bretton woods ski condos for saleWebInvitrogen Hoechst 33342 nucleic acid stain is a popular cell-permeant nuclear counterstain that emits blue fluorescence when bound to dsDNA. This dye is often used to distinguish condensed pycnotic nuclei in apoptotic cells and for cell cycle studies in combination with … country care penngrove

"WebExcitation emission spectrum of Hoechst dye. Both dyes are excited by ultraviolet light at about 350 nm and both emit blue-green fluorescence with a maximum emission spectrum … " - Hoechst excitation

Hoechst excitation

Is the Hoechst 33342 excited at 405nm? ResearchGate

WebNucleic Acid Dyes Max. excitation Max. emission Excitation laser wavelength (nm) wavelength (nm) lines (nm) DAPI 359 461 325,360,405,407 Hoechst 33258 352 461 Hoechst 33342 350 461 SYTOX Blue 431 480 YOYO-1 491 509 SYTOX Green 504 523 TOTO-1, TO-PRO-1 509 533 Mithramycin 450 570 SYTOX Orange 547 570 Chromomycin A3 445 575 … WebThis filter set is designed for optimal detection of DAPI, FITC (fluorescein isothiocyanate), and TRITC (tetramethylrhodamine isothiocyanate) probes when applied in combination, …

Did you know?

WebHoechst 33342 side population (SP) analysis is a common method for identifying stem cells in mammalian hematopoietic and nonhematopoietic tissues. Although widely employed … WebHoechst 33342 nucleic acid stain is a popular cell-permeant nuclear counterstain that emits blue fluorescence when bound to dsDNA. This dye is often used to distinguish condensed …

WebHoechst 33342 is a well-characterized blue-emitting fluorescent compound widely utilized for nuclear staining. Hoechst 33342 permeates cell membranes and binds to the minor … WebThe excitation filter (also called the exciter) transmits only those wavelengths of the illumination light that efficiently excite a specific dye. Although shortpass filter designs were used in the past, bandpass filter …

WebHoechst 33342 is a bis-benzimide derivative that binds to AT-rich sequences in the minor grove of double-stranded DNA. The emission is very bright, and for the purposes of the SP strategy, is detected at two emission wavelengths after UV excitation: Hoechst Red (630–650 nM) and Hoechst Blue (405–450 nM).

WebExcitation Wavelength Range 647 nm Form Liquid Product Line Pierce™ Purity or Quality Grade > 99% Quantity 200 μL Solubility Soluble at working concentrations in water and typical buffers Sub Cellular Localization …

WebThe Nikon dual excitation band DAPI-FITC filter set is designed for the simultaneous detection of the popular fluorophores DAPI and FITC (fluorescein isothiocyanate) when applied in combination, and … country care productshttp://docs.abcam.com/pdf/immunology/fluorochrome_guide.pdf country care rehabilitation and equipmentWebExcitation–emission spectra of Hoechst dyes. Both dyes are excited by ultraviolet light at around 350 nm, and both emit blue-cyan fluorescent light around an emission spectrum … country care shop fayetteville ncWebHoechst 33342 is a bis-benzimide derivative that binds to AT-rich sequences in the minor grove of double-stranded DNA. The emission is very bright, and for the purposes of the SP … country care sunburyWebFeatures of Hoechst 33342 Fluorescent Stain: • Hoechst dye—blue fluorescent stain specific for DNA (i.e., nuclei of eukaryotic cells) • Convenient—provided as an easy-to-use Hoechst … country care pet hospitalWebFluorescein-based dyes and their conjugates have several performance characteristics that may facilitate or limit use in certain applications. Fluorescein displays: A relatively high rate of photobleaching Fluorescent signal that is sensitive to pH changes A relatively broad fluorescence emission spectrum bretton woods ski resort trail mapWebThe wavelength of excitation is critical to the total photons of light that the fluorophore will absorb. Fluorescein isothiocyanate (FITC), for example, will absorb light from 400-530 nm but absorbs most efficiently at its peak or excitation maximum of 490 nm wavelength. bretton woods ski resort lift tickets