Min6 insulin secretion
WebSummary Insulin secretion is essential for maintenance of glucose homeostasis, but the mechanism of insulin granule exocytosis, the final step of insulin secretion, is largely unknown. Here, we investigated the role of Rim2α in insulin granule exocytosis, including the docking, priming, and fusion steps. We found that interaction of Rim2α and Rab3A is … WebThe role of type I diacylglycerol kinases (DGKs) in the regulation of insulin secretion was investigated in MIN6 β-cells. In intracellular Ca2+ concentration ([Ca2+]i) measurement experiments, 1 μM R59949, a type I DGK inhibitor, and 10 μM DiC8, a diacylglycerol (DAG) analog, amplified 22.2 mM glucose-induced [Ca2+]i oscillations in a protein kinase C …
Min6 insulin secretion
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WebThe defect in GSIS was recapitulated in primary islets ex vivo and after TCPTP pharmacological inhibition or lentiviral-mediated TCPTP knockdown in the glucose-responsive MIN6 beta cells, consistent with this being cell autonomous. WebInsulin secretion was determined by enzyme-linked immunosorbent assay, and microfluorimetry with fura-2 was used to examine intracellular Ca (2+) …
WebThis study identified 18 genes that altered glucose-stimulated insulin secretion (GSIS) in pancreatic β-cells, and confirmed Sox11 as a negative regulator of insulin secretion and its role in glucose metabolism. WebNew aspects of the glucose-dependent actions of ERK1/2 in beta cells exerted on cytoplasmic proteins, including synapsin I, and participating in the overall glucose-induced insulin secretion are demonstrated. The p44/p42 Mitogen Activated Protein kinases (ERK1/2) cascade regulates beta cell nuclear events, which modulates cell differentiation …
Web20 jan. 2024 · We have developed a MIN6 subclone (MIN6-6) that allows for high throughput assay of insulin secretion in both population and single cells. In addition, … WebMIN6 cells secrete insulin in response to glucose and other secretagogues, but high passage (HP) MIN6 cells lose their ability to secrete insulin in response to glucose. We …
Web28 jun. 2024 · Introduction. To precisely regulate the blood glucose level 1 – 3, glucose elevation induces Ca 2+ oscillations in pancreatic islet cells that trigger the pulsatile secretion of insulin and glucagon 4 – 7.The oscillatory activity of pancreatic islets is widespread in many species, such as mouse 8 and human 5.Dampening and …
WebMeanwhile, we observed the GLP-1 concentration in serum was increased; GLP-1 is an intestinal glucagon hormone secreted by L cells of the lower digestive tract, and GLP-1 receptor agonists can stimulate the secretion of insulin in a blood glucose-dependent manner, 20 so we speculated that LPC (17:0) improves insulin resistance by activating … efficiency of invertercontent search eacWeb1 nov. 2024 · Glucose-stimulated insulin secretion was higher in the untreated control than other groups and CLW tightly regulated insulin ... B.S.; Jun, W.K. Ginsenosides Rb1 and Rg1 suppress triglyceride accumulation in 3T3-L1 adipocytes and enhance beta-cell insulin secretion and viability in Min6 cells via PKA-dependent pathways ... content search criteriaWeb26 aug. 2024 · VU0119498 Augments ACh-Mediated Increases in Insulin Secretion and Intracellular Calcium Levels in MIN6-K8 Cells. To test the hypothesis that the PAM VU0119498 can promote M3R-mediated insulin secretion, we initially carried out in vitro studies using the MIN6-K8 cell line derived from mouse β cells ( 20 ). efficiency of lead acid batteryWebassayed glucose-stimulated insulin secretion in rodent islets and the insulinoma cell line MIN6. Insulin release from MIN6 cells and rodent islets was significantly inhibited by the PI indinavir with IC 50 values of 1.1 and 2.1 mol/l, respectively. The uptake of 2-deoxyglucose in MIN6 cells was similarly inhibited (IC 50 of 2.0 mol/ efficiency of metasurface antennasWebGlucose-stimulated insulin secretion, glucose transport, glucose phosphorylation and glucose utilization have been characterized in the insulinoma cell line MIN6, which is … content search deleted items office 365Web12 jan. 2024 · MIN6 cells express not only the insulin genes ( Ins1 and Ins2 ), but also the Gcg gene 27, 28. MIN6 cells were shown to secrete GLP-1 in response to glucose. GLP-1 secretion was further... content search date range